SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR
نویسندگان
چکیده
منابع مشابه
Molecular Detection of Rabies by SYBR Green Real Time PCR
The present study was envisaged to compare the sensitivity of SYBR Green real time PCR with immunofluorescence PCR for diagnosis of rabies. SYBR Green real time PCR technique was applied on brain samples collected from 39 rabies suspected animals. Sensitivity of SYBR Green technique was compared in accordance with WHO recommended gold standard test viz. Fluorescent Antibody Technique (FAT) appl...
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The use of a 10-day observation to determine whether a dog is rabid is standard practice. This study was conducted in order to look for evidence of rabies vius in saliva and cerebrospinal fluid (CSF) of suspected live rabid dogs at the time of quarantine by using a SYBR Green real-time RT-PCR based assay for the detection of rabies virus RNA. Saliva and CSF of dogs were collected once on the da...
متن کاملQuantitative detection of chicken meat routine mislabeling in emulsion type sausages and burgers by SYBR green real time PCR assay
ABSTRACT- Today, the authenticity of meat products with less costly and desirable species has increased. Therefore and considering religious, economicalor public health concerns, proper actions should be taken to prevent such frauds. In this study, real time PCR assay was applied for rapid, sensitive and specific identification and quantification of chicken tissue in meat products. Specific pri...
متن کاملIntroducing a New SYBR green Real-time PCR for Detection of SARS-CoV2 Virus Genome
Background and purpose: There are various methods for molecular detection of SARS-CoV2 genome among which, PCR-based methods are the most reliable for making diagnosis. The majority of approved PCR kits for detection of Coronavirus are based on TaqMan real-time PCR which is expensive due to incorporating fluorescent and quencher-harboring probe. The aim of this study was to design a simple and ...
متن کاملDNA quantification using EvaGreen and a real-time PCR instrument.
DNA quantiWcation is an important, frequently used technique, and inaccuracies can result in failures with ligation, restriction, polymerase chain reaction (PCR),1 ampliWed fragment length polymorphism (AFLP), Southern blotting, and other techniques. DNA is most commonly quantiWed using absorbance at 260 nm, but because of the existence of many impurities, this can be an imprecise measurement a...
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ژورنال
عنوان ژورنال: BMC Research Notes
سال: 2011
ISSN: 1756-0500
DOI: 10.1186/1756-0500-4-263